This entry CXIDB-101 contains raw diffraction images collected at SACLA for a paper on the use of grease as a carrier medium. "Viscosity-adjustable grease matrices for serial nanocrystallography" Scientific Reports 10, 1371 (2020) https://doi.org/10.1038/s41598-020-57675-7 Michihiro Sugahara, Koji Motomura, Mamoru Suzuki, Tetsuya Masuda, Yasumasa Joti, Keiji Numata, Kensuke Tono, Makina Yabashi, Tetsuya Ishikawa We encourage you to reprocess this dataset with new programs and ideas. * Datasets This CXIDB entry contains seven datasets. Folders corresponds to the following datasets in Table. 1 of the paper. - kl2_para: Paraffin grease - kl2_s3230: DATPE grease - cellulose: Cellulose - nuclear: Nuclear grease - kl2_para_cryo: Frozen paraffin grease - kl2_para_nano: Paraffin grease (native) - kl2_s3230_nano_pr: DATPE grease (Pr-drivative) The list of file names and checksums is provided in images.md5sum. * Geometry MPCCD contains eight panels (512 x 1024 pixels each). In the HDF5 files, eight panels are stacked vertically to give a 512 x 8192 pixel image for a frame. The detector geometry is provided as CrystFEL geometry files. "sacla-30jan-orig.geom" is the original facility provided geometry. "sacla-30jan-opt.geom" is the refined geometry used for the publication. * Image processing Image files were filtered and written by Cheetah adapted for SACLA. Details of the data processing pipeline are described in the following paper. "Data processing pipeline for serial femtosecond crystallography at SACLA." Nakane, Takanori, et al. Journal of applied crystallography 49.3 (2016). The images are ready for processing by CrystFEL. For example: cellulose, kl2_para, kl2_para_cryo: indexamajig -i files.lst -o output.stream \ -g sacla-30jan-opt.geom --peaks=peakfinder8 \ --threshold=0 --min-gradient=200000 --min-snr=5 --int-radius=3,4,7 \ --indexing=dirax -p prok.cell kl2_para_nano, kl2_s3230_nano_pr: indexamajig -i files.lst -o output.stream \ -g sacla-30jan-opt.geom --peaks=peakfinder8 \ --threshold=0 --min-gradient=200000 --min-snr=5 --int-radius=3,4,7 \ --tolerance=5,5,3,1.5 --indexing=asdf -p prok.cell kl2_s3230: indexamajig -i files.lst -o output.stream \ -g sacla-30jan-opt.geom --peaks=peakfinder8 \ --threshold=0 --min-gradient=200000 --min-snr=5 --int-radius=3,4,7 \ --tolerance=5,5,3,1.5 --indexing=dirax -p prok.cell nuclear: indexamajig -i files.lst -o output.stream \ -g sacla-30jan-opt.geom --peaks=zaef \ --threshold=0 --min-gradient=200000 --min-snr=5 --int-radius=3,4,7 \ --indexing=asdf -p prok.cell * Contact Questions should be sent to Michihiro Sugahara, whose mail address is shown in the CXIDB.