This deposition contains raw diffraction images collected at SACLA for the following paper. "Crystal structure of CmABCB1 multi-drug exporter in lipidic mesophase revealed by LCP-SFX" Dongqing Pan, Ryo Oyama, Tomomi Sato, Takanori Nakane, Ryo Mizunuma, Keita Matsuoka, Yasumasa Joti, Kensuke Tono, Eriko Nango, So Iwata, Toru Nakatsu and Hiroaki Kato IUCrJ (2022), doi: 10.1107/S2052252521011611 We encourage you to reprocess this dataset with new programs and ideas. We are happy to hear from you if you manage to solve the structures to higher resolution. * Datasets This deposition contains four SFX datasets mentioned in the paper: - E20: LCP microcrystals (10 % HEC) - E25: LCP microcrystals (12.5 % HEC) - M25-Wed: LCP microcrystals (12.5 % HPMC) - M30-Wed: LCP microcrystals (17.5 % HPMC) Checksums are provided in respective folders. * Geometry MPCCD contains eight panels (512 x 1024 pixels each). In the HDF5 files the eight panels are stacked vertically to give a 512 x 8192 pixel image for a frame. The detector geometry is provided as CrystFEL geometry files. sacla-15nov-500-original.geom is the initial, non-optimized metrology based on facility provided values. Others are refined geometries used for final processing. * Processed stream file We also uploaded CrystFEL stream files in the Processed directory. * Image processing Image files were written by Cheetah adapted for SACLA. Details of this pipeline were published in https://doi.org/10.1107/S1600576716005720. The images are ready for processing by CrystFEL. E20: indexamajig -i E20.lst -o E20.stream -j 14 -g E20-sacla-15nov-503-opt2.geom -p ../sfx.cell --indexing=xgandalf \ --peaks=peakfinder8 --threshold=100 --min-snr=4.5 --min-pix-count=2 --local-bg-radius=3 --int-radius=3,4,7 E25: indexamajig -i E25.lst -o E25.stream -j 14 -g E25-sacla-15nov-501-opt2-pr.geom -p ../sfx.cell --indexing=xgandalf \ --peaks=peakfinder8 --threshold=0 --min-snr=4.5 --min-pix-count=2 --local-bg-radius=3 --int-radius=3,4,7 M25-Wed: indexamajig -i M25-Wed.lst -o M25-Wed.stream -j 14 -g M25Wed-sacla-15nov-498-opt2-pr.geom -p ../sfx.cell \ --indexing=xgandalf --peaks=peakfinder8 --threshold=100 --min-snr=4.5 --min-pix-count=2 --local-bg-radius=3 --int-radius=3,4,7 M35-Wed: indexamajig -i M35-Wed.lst -o M35-Wed.stream -j 14 -g M35Wed-sacla-15nov-499-opt2-pr.geom -p ../sfx.cell \ --indexing=xgandalf --peaks=peakfinder8 --threshold=100 --min-snr=4.5 --min-pix-count=2 --local-bg-radius=3 --int-radius=3,4,7 These are parameters we used with CrystFEL 0.9.0. Best parameters for newer versions of CrystFEL are probably different. A dxtbx module necessary for processing with DIALS is also available. See https://github.com/biochem-fan/cheetah/wiki for detailed explanations and tutorials. * Contact Questions about data processing should be sent to Takanori Nakane. Other questions should be sent to Toru Nakatsu. Mail addresses are shown in the CXIDB.