This deposition contains raw diffraction images collected at SACLA 
for the following paper.

"High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure"
Yoshiaki Shimazu, Kensuke Tono, Tomoyuki Tanaka, Yasuaki Yamanaka, Takanori Nakane, Chihiro Mori,
Kanako Terakado Kimura, Takaaki Fujiwara, Michihiro Sugahara, Rie Tanaka, R. Bruce Doak, Tatsuro Shimamura,
So Iwata, Eriko Nango, Makina Yabashi
Journal of Applied Crystallography (2019), doi: 10.1107/S1600576719012846

We encourage you to reprocess this dataset with new programs and ideas.

* Datasets

This CXIDB entry contains a dataset for lysozyme.
The other dataset for A2a receptor is in CXIDB-117.

* Geometry

MPCCD contains eight panels (512 x 1024 pixels each). In the HDF5 files,
eight panels are stacked vertically to give a 512 x 8192 pixel image
for a frame.

The refined detector geometry is provided as a CrystFEL geometry file, refined.geom.
The geometry file before optimisation is original.geom.

* Image processing

Image files were filtered and written by Cheetah adapted for SACLA. 
Details of the data processing pipeline are described in the following paper.

"Data processing pipeline for serial femtosecond crystallography at SACLA." 
Nakane et al. Journal of applied crystallography 49.3 (2016).

See also tutorials at https://github.com/biochem-fan/cheetah/wiki.

This format is DIFFERENT from SACLA HDF5 format in the Hg-SIRAS dataset (CXIDB #31).
In contrast to the SIRAS dataset, dark current subtraction and relative gain
correction have already been applied. Here, one photon corresponds to 10 counts in 
the file. In other words, the gain is 10.

The images are ready for processing by CrystFEL.

A dxtbx module necessary for processing with cctbx.xfel is under development.
It is already in the cctbx repository but you have to edit some parameters
(e.g. camera length). Ask Takanori for details.

* Contact

Please contact to Eriko Nango, whose mail address is shown in the CXIDB.