This dataset contains two sets of X-ray diffraction images recorded from a stream of lysozyme crystals in a flow-focussing microfluidic device at beamline MASSIF-3 (ID30A-3) at the ESRF (Grenoble, France). Coordinates are available in the PDB as 6RXI. https://doi.org/10.2210/pdb6RXI/pdb For further details: Monteiro et al., 3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow, IUCrJ 7, 207-219 (2020). https://doi.org/10.1107/S2052252519016865 Two datasets were recorded at 3 mm and 6 mm, respectively, beyond the channel junction. The following files are available for download: ## 3 mm lyso_large_0p5PEG_CTO_3mm_1_1_master.h5 lyso_large_0p5PEG_CTO_3mm_2_1_master.h5 lyso_large_0p5PEG_CTO_3mm_3_1_master.h5 lyso_large_0p5PEG_CTO_3mm_4_1_master.h5 lyso_large_0p5PEG_CTO_3mm_5_1_master.h5 lyso_large_0p5PEG_CTO_3mm_6_1_master.h5 lyso_large_0p5PEG_CTO_3mm_*_1_data_??????.h5 (6000 files) lyso_large_0p5PEG_CTO_3mm_all_raw_th40_gr100_xgandalf_v080.stream mask_badpixels.h5 ## 6 mm lyso_large_0p5PEG_CTO_6mm_1_1_master.h5 lyso_large_0p5PEG_CTO_6mm_2_1_master.h5 lyso_large_0p5PEG_CTO_6mm_4_1_master.h5 lyso_large_0p5PEG_CTO_6mm_5_1_master.h5 lyso_large_0p5PEG_CTO_6mm_6_1_master.h5 lyso_large_0p5PEG_CTO_6mm_7_1_master.h5 lyso_large_0p5PEG_CTO_6mm_*_1_data_??????.h5 (6000 files) lyso_large_0p5PEG_CTO_6mm_all_raw_th40_gr100_xgandalf_v080.stream mask_badpixels.h5 Please note: - Each lyso_*_data_??????.h5 file contains 100 diffraction images. - The X-ray beam was switched off/on every ~300 ms (~60 images) in order to limit the production of X-ray induced hydrogen bubbles, without interrupting the recording of X-ray images. Therefore, about half of the diffraction images are empty, but thanks to efficient compression, this does (almost) not increase the size of the final dataset. - The HDF5 metadata indicates continuous omega rotation during the datasets, because the goniometer was constantly rotating and in fact triggered the recording of diffraction images, but the microfluidic device was not attached to the goniometer, and therefore these omega angles are meaningless. Contacts: Diana C.F. Monteiro David von Stetten