Deposition Summary
Depositor: Gijs van der Schot
Deposition date: 2015-02-10
Last modified: 2015-02-10
DOI: 10.11577/1169686
Publication Details
Title: Imaging single cells in a beam of live cyanobacteria with an X-ray laser
Authors: Gijs van der Schot et al.
Journal: Nature Communications
Year: 2015
DOI: 10.1038/ncomms6704
Experimental Conditions
Method: Single Particle X-ray Diffraction Imaging
Sample: Cyanobium gracile
Wavelength: 2.398 nm
Lightsource: LCLS
Beamline: AMO
Data Files
Diffraction Patterns: cxidb-26.cxi
Auxiliary Files
Hawk Configuration: uwrapc.conf
Reconstruction Script:


This entry contains ten diffraction patterns, and reconstructions images, of individual living Cyanobium gracile cells, imaged using 517 eV X-rays from the LCLS XFEL. The Hawk software package was used for phasing. The Uppsala aerosol injector was used for sample injection, assuring very low noise levels. The cells come from various stages of the cell cycle, and were imaged in random orientations.