Deposition Summary
Depositor: Gijs van der Schot
Deposition date: 2015-02-10
Last modified: 2015-02-10
DOI: 10.11577/1169687
Publication Details
Title: Imaging single cells in a beam of live cyanobacteria with an X-ray laser
Authors: Gijs van der Schot et al.
Journal: Nature Communications
Year: 2015
DOI: 10.1038/ncomms6704
Experimental Conditions
Method: Single Particle X-ray Diffraction Imaging
Sample: Synechococcus elongatus
Wavelength: 1.127 nm
Lightsource: LCLS
Beamline: AMO
Data Files
Diffraction Pattern: cxidb-27.cxi


Diffraction pattern of a micron-sized S. elongatus cell at 1,100 eV photon energy (1.13 nm wavelength) with ~10^11 photons per square micron on the sample in ~70 fs. The signal to noise ratio at 4 nm resolution is 3.7 with 0.24 photons per Nyquist pixel. The cell was alive at the time of the exposure. The central region of the pattern (dark red) is saturated and this prevented reliable image reconstruction.