CXIDB ID 171 | |
Deposition Summary | |
---|---|
Depositor: | Keitaro Yamashita |
Contact: | [email protected] |
Deposition date: | 2020-10-21 |
Last modified: | 2020-11-05 |
DOI: | 10.11577/1679953 |
Associated PDB: | 7CJ3 |
Publication Details | |
Title: | Structural insights into the mechanism of rhodopsin phosphodiesterase |
Authors: | Tatsuya Ikuta et al. |
Journal: | Nature Communications |
Year: | 2020 |
DOI: | 10.1038/s41467-020-19376-7 |
Experimental Conditions | |
Method: | Synchrotron Crystallography (small-wedge) |
Sample: | RhPDE-TMD |
Wavelength: | 1.00 Å (12.4 keV) |
Lightsource: | SPring-8 |
Beamline: | BL32XU |
Data Files | |
Auxiliary Files | |
Raw Data: | 180608_BL32XU/ (4 KB) |
Raw Data: | 180803_BL32XU/ (4 KB) |
Description
Crystal structures of TMD of Rhodopsin phosphodiesterase (PDB code: 7CJ3). 6160 small-wedge (10°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm2 and oscillation step was 0.1°. The crystals belonged to space group P212121 with unit cell parameters a~66, b~74, c~117 Å. 864 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 271 integrated results were merged at 2.6 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result.